Direct Immobilisation of DNA Probes for the Development of Affinity Biosensors

Ilaria Mannelli 1,  Sara Tombelli 1,  Maria Minunni 1,  Ronghui Wang 1,2,  Marco Mascini 1

1. Dipartimento di Chimica, Università di Firenze (UNIFI), Via della Lastruccia 3, Sesto Fiorentino (Firenze) 50019, Italy
2. Central South University, Chemistry & Chemical Engineering College, Changsha, Hunan 410082, China

Abstract

An immobilisation procedure based on the direct coupling of thiol-derivatised oligonucleotide probes (Probe-C6-SH) to bare gold sensor surfaces has been optimised for DNA sensing applications. The instrumentation used were the commercially available instruments BIACORE XTM and SPREETATM based on surface plasmon resonance (SPR) and a quartz crystal microbalance (QCM).
The performances of the DNA-based sensors resulting from direct coupling of thiol-derivatised DNA probes onto gold SPR-chips or electrodes of quartz crystals, have been studied in terms of the main analytical parameters, i.e. selectivity, sensitivity, reproducibility, analysis time, etc.
A comparison between the thiol-derivatised immobilisation approach and a reference immobilisation method, based on the coupling of biotinylated oligonucleotide probes onto a streptavidin coated dextran sensor surface, using synthetic complementary oligonucleotides has been discussed in detail.
The DNA biosensors developed have been also used to test DNA samples amplified by polymerase chain reaction (PCR).

Legal notice
  • Legal notice:

    Copyrighted materials, (c) Pielaszek Research, all rights reserved.
    The above materials, including auxiliary resources, are subject to Publisher's copyright and the Author(s) intellectual rights. Without limiting Author(s) rights under respective Copyright Transfer Agreement, no part of the above documents may be reproduced, stored in or introduced into a retrieval or caching system, or transmitted in any form or by any means (electronic, mechanical, photocopying, recording or otherwise), or for any purpose, without the express written permission of Pielaszek Research, the Publisher. Express permission from the Author(s) is required to use the above materials for academic purposes, such as lectures or scientific presentations.
    In every case, proper references including Author(s) name(s) and URL of this webpage: http://science24.com/paper/1266 must be provided.

 

Related papers
  1. Aligned carbon nanotube thin films for genosensor development
  2. A new class of receptors for the development of biosensors: aptamers for clinical bio-markers

Presentation: Short communication at SMCBS 2003 Workshop, by Ilaria Mannelli
See On-line Journal of SMCBS 2003 Workshop

Submitted: 2003-10-21 16:42
Revised:   2009-06-08 12:55
Google
 
Web science24.com
© 1998-2021 pielaszek research, all rights reserved Powered by the Conference Engine