Fluorescent Polymer Biosensor for Nucleotides

Alina K. Mielniczak ,  Barbara J. Wandelt 

Technical University of Łódź, Department of Molecular Physics (TUL), Zeromskiego 116, Łódź 90-924, Poland

Abstract

Progress of industrial processes and medical diagnostics increases need for reliable and cheap instrumentation for continuous recording of data in real time, without complicated sampling. Optical sensor technology can provide low-cost devices that can be tuned to a wide field of applications by coating a fibre optic with a chemically sensitive thin-layer polymer [1].
The molecular imprinting is a technology which produce a polymer with selectively binding sites. This polymer has the ability to recognize a molecule by selective adsorption on a microporous matrix. This technology involves polymerisation of functional monomers together with template molecule. Than the template molecule is removed leaving behind functionalised sites that are able to recognize the template [2].
We developed imprinted polymer with both the recognition element together with transducer element, which generates fluorescence of which intensity depends upon the template binding. A fluorescent functional monomer, trans-4-(p,N,N-dimethyloaminostyryl)-N-vinylbenzylpyridinium chloride (vbDMASP), has been used together with a conventional functional monomers to prepare the polymer using imprinting technology [3], the recognition sites were prepared against the nucleotides.
We report here our studies on a thin-layer fluorescent imprinted polymer. Both the steady-states and time-resolved fluorescence spectroscopy shown quenching of fluorescence of the imprinted polymer when it was incubated in presence of nucleotides.

This project was supported by the KBN grant No. 4 T09B 101 24.

References
1. R. C. Hughes, A. J. Ricco, M. A. Butler, S. J. Martin, Science 254 (1991) 74.
2. A. Mayes, K. Mosbach, Trends in Analytical Chemistry, vol. 16, No. 6, 1997, 321.
3. B. Wandelt, P. Turkewitsch, S. Wysocki , G. D. Darling, Polymer 43 (2002) 2777.

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Presentation: Short communication at SMCBS 2003 Workshop, by Alina K. Mielniczak
See On-line Journal of SMCBS 2003 Workshop

Submitted: 2003-10-14 14:53
Revised:   2009-06-08 12:55
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