Microelectronic multi-parametrical enzymatic biosensor

Nickolaj F. Starodub 1Olexandra M. Shmireva 2

1. Ukrainian National Academy of Sciences, A. V. Palladin Institute of Biochemistry, 9 Leontovicha, Kyiv 01030, Ukraine
2. National Technical University of Ukraine KPI, pr.Peremogy 37, Kyiv 03056, Ukraine

Abstract

The measurement of one parameter at the complicate and multifactor character of biochemical processes could not in principal allow to provide creation of biosensor which will correspond to full complex of practice demands including selectivity, sensitivity, stability in general and to contaminated substances, repetition of results, needed time of functioning and others. To achieve this purpose it was created microelectronic enzymatic biosensor which gives possibility to register number of parameters at the determination of glucose, sucrose and lactose. Such integral biosensor contents simultaneously two different channels: amperometrical and thermometrical. Amperometrical channel includes three planar microelectrodes and their registered results are presented as average value. Thermometrical channel is presented by thin layer sensors which work in differential regime. Output signals of the integral biosensor are processed by the special electronic block. Thin layer temperature sensors were prepared by two ways: on the basis of platinum or oxide cerium. These sensors together with the platinum working electrodes and reference electrode (AgCl) for the amperometrical measuring were situated on the common solid phase. Three working electrodes were taken for the increasing of accuracy of measurements. Thermometrical channel contents also working and reference thermistors which had ophidian form. The sensitive layer was formed in three steps: deposition of polyvinylchloride, mixture of one of enzyme with glutaraldehyde and, at last, nitrocellulose. The sensitivity (g) of the biosensor was determined according to the next equation: where - DI and DN – changes of current in nA and analyzed substance, respectively. The main it characteristics are presented bellow (see Tabl.). In general it is necessary to mention that they correspond to the

Analytes

Way of analysis

Controlled concentrations, мМ

Sensitivity

Glucose

Thermometry

0,002-25

2,35 rel.units/mМ

Amperometry

0,5-50

4,3 нА/mМ

Sucrose

Thermometry

0,005-100

0,45 rel.units /mМ

Amperometry

0,1-80

3,3 нА/mМ

Lactate

Thermometry

0,1-10

0,85 rel.units /mМ

Amperometry

0,1-200

0,9 нА/mМ

practice demand in respect the sensitivity from one side and from other one the obtained values have double conformation. We believe that the proposed approach will find perspective development at the creation of analytical devices for control of different biochemical quantities.

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Presentation: Keynote lecture at SMCBS'2007 International Workshop, by Nickolaj F. Starodub
See On-line Journal of SMCBS'2007 International Workshop

Submitted: 2007-06-25 11:08
Revised:   2009-06-07 00:44
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