Studies of New Procedures for Sensors and Biosensors Development Based on Modified Gold and Platinum Supports

Simona Carmen F. Litescu 1Camelia Bala 1Jean-Louis Marty 2

1. University of Bucharest, Faculty of Chemistry, Panduri 90, Bucharest, Romania
2. Centre de Phytopharmacie, Universite Perpignan, Perpignan, France

Abstract

Possible new procedures devoted to sensor and biosensors development using deposed layers on solid supports were studied, the characteristics of the resulted systems and their potential applications being presented.
Solution casting, spin coating and electrochemical treatments were studied and compared as possible deposition procedures. Advantages and drawbacks of each used method were pointed out as result of the carried out studies oriented to two different aims: enzyme immobilisation, respectively the development of a potential electrochemical model for antioxidants properties evaluation.
Studies dedicated to enzymes immobilisation initiated with the study of self-assembly structuring of several well-known mercapto-derivatives (glutathion, cysteamine, 16-mercaptodecanoic acid) on solid supports, and, as result of the obtained data, reached to the deposition of mercapto-silane derivatives (3-aminopropyl triethoxy silane-3APTS, and 3-mercapto-trimethoxy silane- 3 MTMSy) on the same kind of supports. Electrochemical, spectrochemical (Fourier Transform Infrared) and Scanning Electron Microscopy characterisations were performed. An optimum procedure, able to ensure a good degree of deposition reproducibility for 3 ammino-propyltrietoxysilane and 3-mercaptopropyl trimethoxysilane was established. The deposed layer was electrochemical, FTIR and SEM characterised proving homogenous distribution of functional groups on layer surface.
Potential applications of the developed system to acetylcholinesterase immobilisation were considered.
A LDL- gold modified electrode was developed and was used as lipidic model for the properties assessment of two of the most commonly used phenol-like preservatives, the deposed surface being characterised. The resulted tool was applied to antioxidative potentiality monitoring of investigated compounds in bulk system using two sources of free radical attack, other possible applications being envisaged.

 

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Presentation: Keynote lecture at SMCBS 2003 Workshop, by Simona Carmen F. Litescu
See On-line Journal of SMCBS 2003 Workshop

Submitted: 2003-09-11 23:00
Revised:   2009-06-08 12:55