Optimization of culture medium for laccase production by Trametes versicolor

Anna Matuszewska 1Jerzy Rogalski Magdalena Szwarc 

1. Maria Curie Sklodowska University, Department of Biochemistry, Pl. Sklodowskiej 3, Lublin 20-031, Poland

Abstract

White-rot Basidiomycetes are mostly known for their capability to effective ligninolysis. The best known lignin destructors include a Trametes versicolor, a potent producer of laccase and peroxidases, oxidases mainly responsible for the lignin polymer degradation. Laccases (benzenediol:oxygen oxidoreductases; EC 1.10.3.2.), multicopper enzymes belonging to the blue oxidases, catalyze the one-electron oxidation of a wide variety of organic and inorganic substrates, including mono-, di- and polyphenols, aminophenols, methoxyphenols, aromatic amines and ascorbate, with the concomitant four-electron reduction of oxygen to water.

The effects of the carbon and nitrogen sources, initial pH and concentration of Cu(II) on laccase production by Trametes versicolor were tested.

All experiments were carried out in 48-wells ELISA plates containing 1,5 ml culture medium each well. The plates were incubated at 28οC on a shaker. Laccase activity was determined as the increase in absorbance at 515 nm due to the oxidation of syringaldazine. Protein concentration in the culture filtrate was determined by using Folina-Ciocalteu’a reagent and bovine serum albumin as the standard.

Analysis showed that 2,5 g/l glucose, 0,5 g/l asparagines, an initial pH of 6,0 were the optimal conditions for laccase production. When using an optimized medium and stimulating enzyme formation by the addition of 2,5 mM Cu, maximal laccase activities obtained in a cultivation were approximately 740 nkat/l.

 

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Presentation: Poster at Zjazd Polskiego Towarzystwa Biochemicznego, Sympozjum M, by Anna Matuszewska
See On-line Journal of Zjazd Polskiego Towarzystwa Biochemicznego

Submitted: 2007-05-06 00:10
Revised:   2009-06-07 00:44