Cell proliferation and DNA repair were studied in sheep lymphocytes obtained from peripheral blood of animals infected with bovine leukemia virus (BLV) which was confirmed by serological tests. Lymphocytes were cultivated in RPMI 1640 medium supplemented with antibiotics and mitogens. Cultures in vitro were treated with benzo(α)pyrene diol-epoxide (BPDE) and aflatoxin B1 (AFB1) in range of different concentration. Cell proliferation was determined by incorporation of 3H-thymidine (3H-TdR) into cultivated cells and DNA repair was estimated by incorporation of 3H-TdR into DNA of hydroxyurea insensitive lymphocytes. Proviral DNA integrated with host cells stimulated infected lymphocytes 2.5 times higher than normal cells, however, lymphocytes treated with mitogens increased proliferation up to 1000 time higher. Normal lymphocytes treated with BPDE or AFB1, showed decreased proliferation and DNA repair according to applied doses. Lymphocytes from BLV infected animal showed lower ability to repair DNA damage made by both toxins. The highest differences were statistically significant (p<0.05) for BPDE at concentration 1.0 – 1.5 μM/ml and for AFB1 at concentration 0.5 – 1.0 μM/ml. In conclusion we can say, that oncogenic virus BLV is able to decreased ability of cells to mount defense mechanism against exposure to BPDE and AFB1, xenobiotics with evident genotoxic properties.

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