Diamine biomolecules surface functionalization single-walled carbon nanotube as gene-delivery vector

Chia-Yu Li 1Chung-King Hsu Feng-Huei Lin Leszek Stobinski 2

1. Natinal Taipei University of Technology (NTUT), 1, Sec. 3, Chung-Hsiao E. Rd.,, Taipei 106, Taiwan
2. Polish Academy of Sciences, Institute of Physical Chemistry, Kasprzaka 44/52, Warszawa 01-224, Poland

Abstract

The development of new and stable gene carrier is of great importance to improve the tranfection efficiency and achieve to certain fundamental needs in gene therapy such as therapeutic protein formation, immunogenicity enhancement and apoptosis induction. The stability and applicability of the system which contains single-walled carbon nanotube (SWNCT) to combine with certain sequence of plasmid DNA(EGFp-C1) were examine by computer simulation. Raman spectroscopy was used as supporting information to verify the process and phenomenon of complex formation. Two diamine molecules, 1,4-diaminobutane and polyoxyethylene bis-amine, were applied in order to load positive charges onto the surface of SWCNT under physiology condition and also enhance the binding ability of CNT to EGFp-C1. The formation of peptide bond was examined with FTIR to confirm the success crossliking. The amount of surface functional groups was checked out with TGA, which found that they are at an extremely low concentration but show great influence on the physical property. Biocompatibility was evaluated with LDH, MTT and total DNA. The results show a significant increase in cell viability and number for SWCNT-1,4-diaminobuatne complex. IL-6 release was measure to justify the inflammatory inducing and give the hint of translocation mechanism. The binding efficiency of functionalized SWCNT to EGFp-C1 was analyzed through the binding strength under electrophoresis.In-vitro transfection was also done to justify the transfection efficiency but showing no significant increase compare with other non-viral vectors which may due to the binding force of such complex.

Keywords: gene carrier, single-walled carbon nanotube, 1,4-diaminobutane, polyoxyethylene bis -amine, biocompatibility


Legal notice
  • Legal notice:

    Copyright (c) Pielaszek Research, all rights reserved.
    The above materials, including auxiliary resources, are subject to Publisher's copyright and the Author(s) intellectual rights. Without limiting Author(s) rights under respective Copyright Transfer Agreement, no part of the above documents may be reproduced without the express written permission of Pielaszek Research, the Publisher. Express permission from the Author(s) is required to use the above materials for academic purposes, such as lectures or scientific presentations.
    In every case, proper references including Author(s) name(s) and URL of this webpage: http://science24.com/paper/10588 must be provided.

 

Related papers
  1. Carbon nanotubes applications for drug and gene delivery
  2. Oczyszczanie i modyfikacja nanomateriałów węglowych
  3. Carbon Nanotubes Supported Bimetallic Palladium-Gold Electrocatalysts for Direct Formic Acid Fuel Cell
  4. Raman spectroscopy studies of metal - carbon nanotubes composite materials.
  5. Laser radiation-induced effects in Raman spectra of multiwalled carbon nanotubes
  6. Carbon Nanotubes: Modifications and Applications
  7. Catalytic Activity and Selectivity of Pt/MWCNTs System in Hydrodechlorination of Freon CFC-12
  8. Ferrimagnetic DP-Bioglass Particles Modified with Polyethylene Glycol and Folic Acid Used for Drug Delivery Process
  9. Metal oxide hybrid UV-curable painting for anti-static film

Presentation: Oral at E-MRS Fall Meeting 2007, Acta Materialia Gold Medal Workshop, by Leszek Stobinski
See On-line Journal of E-MRS Fall Meeting 2007

Submitted: 2007-04-06 19:04
Revised:   2009-06-07 00:44
Google
 
Web science24.com
© 1998-2022 pielaszek research, all rights reserved Powered by the Conference Engine